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This model train set, composed of a red #95 4-4-0 Civil War-era steam tender locomotive, several passenger cars and boxcars, and a caboose, makes an appearance during one of Andy's playtime fantasies at the very beginning of Toy Story 3.
Toy Story 3
The train was seen traveling through a canyon in a desert carrying some money and "orphans" (trolls) when all of a sudden One-Eyed Bart (Mr. Potato Head), his wife One-Eyed Betty (Mrs. Potato Head), and their kids (the Aliens) raid the train while driving Barbie's pink convertible, only to be confronted by Woody and Jessie on Bullseye. While the heroes and villains fight each other with the former using their dinosaur (Rex) against the latter's force-field attack dog (Slinky Dog), The Evil Dr. Porkchop (Hamm) uses his spaceship's laser to destroy the bridge the train was on, only for it to be saved by Buzz Lightyear when Bart hijacks the ship and drops a "Death by Monkeys" on the heroes...