- Fillmore: "Hey, Luigi. Which way to the hotel, man?"
Luigi: "What? No friend of mine will stay in a hotel in-a my village. You will stay with my...
- —Luigi introduces Uncle Topolino to his friends.
In the small village of Carsoli, near Porto Corsa, Italy, Luigi’s favorite uncle, Uncle Topolino, resides with his beloved wife, Mama Topolino. Uncle Topolino is a 1937 Fiat 500 "Topolino" and the owner of the village’s tire shop, Topolino's Tires, where he taught Luigi and Guido everything they know, though Uncle Topolino is full of sage advice about more than just tires.
In the movie, he greets Team Lightning McQueen. He then says that he can tell that McQueen is sad, but he states later that Luigi told him. McQueen tells Topolino his problem of being mean to Mater and Mater leaving. He tells McQueen some wise words of wisdom. He tells him that Luigi and Guido used to always fight, but they always resolved their problems. After strolling off with Mama Topolino, he is not seen for the rest of the movie.
Topolino is kind and nice, and loves to help out. This is parodied in the trailer "V12 TV" when he takes the name of "Dr. Fill it Up", a parody of Dr. Phil.
- Topolino is a tribute to the Fiat 500 "Topolino" as well as a Disney in-joke: Topolino ("little mouse") is Mickey's name in Italian.
- Uncle Topolino is a 1937 Fiat 500 "Topolino".
- Uncle Topolino along with Mama Topolino are some of the very few characters with no turntable video.
- According to his die-cast, his license plate reads GE-18 31A6.
- Uncle Topolino: "Hey, race car! You look so down, so low. Is like you have flat tires."
Mama Topolino: (something in Italian)
Uncle Topolino: "She said you look like you are starving, that she's gonna make you a big meal and fatten you up."
Lightning McQueen: "Oh, no. Uh, Mama Topolino, please, you don't need to make a fuss. . ." (sighs as Mama Topolino drives into the building)
Uncle Topolino: "Capisco [I see]. I understand. Is a problem, yes, between you and a friend?"
Lightning McQueen: "How did you know that?"
Uncle Topolino: "A wise car hears one word and understands two... That, and Luigi told me. While Mama cooks, come and take a stroll with me."
Lightning McQueen: "I brought my friend, Mater, along on the trip, and I told him he needed to act different, that we weren't in Radiator Springs."
Uncle Topolino: "This Mater is a close friend?"
Lightning McQueen: "He's my best friend."
Uncle Topolino: "Then why would you ask him to be someone else?"
Lightning McQueen: (thinking for a moment) "What did I do? I said some things during our fight."
Uncle Topolino: "You know, back when Guido and Luigi used to work for me, they would fight over everything. They fight over what Ferrari was the best Ferrari, which one of them looked more like a Ferrari. There were even some non-Ferrari fights. So I tell them, 'Va bene [It's okay], it's okay to fight. Everybody fights now and then, especially best friends. But you got to make up fast. No fight more important than friendship.'" (during what Uncle Topolino said, Luigi and Guido were fighting over who should dance with the girl, then another girl came, and they danced one with one girl)
Uncle Topolino: (something in Italian)
Lightning McQueen: "What does that mean?"
Mama Topolino: "'Whoever find a friend, find a treasure.' Now, mangia [eat]. Eat!"
(Uncle Topolino and Mama Topolino drive away, arguing in Italian)
- —Uncle Topolino gives Lightning some wise advice.